Deepdof-se: Affordable Deep-learning Microscopy Platform For Slide-free Histology

DeepDOF-SE is a deep-learning-enabled microscope that can rapidly scan intact tissue at cellular resolution without the need for physical sectioning. It uses inexpensive vital fluorescent dyes and a deep-learning algorithm to extend the depth-of-field, allowing for rapid acquisition of in-focus images from large areas of tissue. A semi-supervised generative adversarial network virtually stains the images, making it a rapid and affordable slide-free histology platform for intraoperative tumor margin assessment and in low-resource settings.