Artificial intelligence (AI) is a type of computer science that develops software programs for intelligent execution of tasks or decision making. AI has demonstrated capabilities for the resolution of a variety of biomedical problems, including cancer, over the past decade. Machine learning and deep learning are subfields of AI that apply mathematical and statistical algorithms to improve computer performance. AI can play an essential role in a wide variety of aspects of oncology, such as tumor detection and segmentation, histopathological diagnosis, tracking tumor development, clinical decision making, cancer therapy development and validation, and prognosis prediction. This Topical Collection will highlight several of the above issues and welcomes submissions of research and review articles addressing several facets of AI in both basic and clinical cancer research.